6BGC

The crystal structure of the W145A variant of TpMglB-2 (Tp0684) with bound glucose

6BGC の概要

• エントリーDOI: 10.2210/pdb6bgc/pdb
• 分子名称: Glucose/galactose-binding lipoprotein, beta-D-glucopyranose, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: glucose, syphilis, sugar binding protein
• 由来する生物種: Treponema pallidum (strain Nichols)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 83889.24

構造登録者

Brautigam, C.A.,Norgard, M.V.,Deka, R.K. (登録日: 2017-10-27, 公開日: 2018-01-31, 最終更新日: 2023-10-04)

主引用文献

Brautigam, C.A.,Deka, R.K.,Liu, W.Z.,Norgard, M.V.
Crystal structures of MglB-2 (TP0684), a topologically variant d-glucose-binding protein from Treponema pallidum, reveal a ligand-induced conformational change.
Protein Sci., 27:880-885, 2018

PubMed Abstract: Previously, we determined the crystal structure of apo-TpMglB-2, a d-glucose-binding component of a putative ABC transporter from the syphilis spirochete Treponema pallidum. The protein had an unusual topology for this class of proteins, raising the question of whether the d-glucose-binding mode would be different in TpMglB-2. Here, we present the crystal structures of a variant of TpMglB-2 with and without d-glucose bound. The structures demonstrate that, despite its aberrant topology, the protein undergoes conformational changes and binds d-glucose similarly to other Mgl-type proteins, likely facilitating d-glucose uptake in T. pallidum.

PubMed: 29318719
DOI: 10.1002/pro.3373

実験手法

X-RAY DIFFRACTION (2.082 Å)