6BA8

YbtT - Type II thioesterase from Yersiniabactin NRPS/PKS biosynthetic pathway

6BA8 の概要

• エントリーDOI: 10.2210/pdb6ba8/pdb
• 関連するPDBエントリー: 6BA9
• 分子名称: Iron aquisition yersiniabactin synthesis enzyme, YbtT (2 entities in total)
• 機能のキーワード: thioesterase, non-ribosomal peptide synthesis, sideraphore synthesis, yersiniabactin, hydrolase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32787.04

構造登録者

Brett, T.J.,Kober, D.L.,Ohlemacher, S.I.,Henderson, J.P. (登録日: 2017-10-12, 公開日: 2018-10-31, 最終更新日: 2023-10-04)

主引用文献

Ohlemacher, S.I.,Xu, Y.,Kober, D.L.,Malik, M.,Nix, J.C.,Brett, T.J.,Henderson, J.P.
YbtT is a low-specificity type II thioesterase that maintains production of the metallophore yersiniabactin in pathogenic enterobacteria.
J. Biol. Chem., 293:19572-19585, 2018

PubMed Abstract: Clinical isolates of , , and frequently secrete the small molecule metallophore yersiniabactin (Ybt), which passivates and scavenges transition metals during human infections. YbtT is encoded within the Ybt biosynthetic operon and is critical for full Ybt production in bacteria. However, its biosynthetic function has been unclear because it is not essential for Ybt production by the reconstituted nonribosomal peptide synthetase/polyketide synthase (NRPS/PKS) pathway. Here, we report the structural and biochemical characterization of YbtT. YbtT structures at 1.4-1.9 Å resolution possess a serine hydrolase catalytic triad and an associated substrate chamber with features similar to those previously reported for low-specificity type II thioesterases (TEIIs). We found that YbtT interacts with the two major Ybt biosynthetic proteins, HMWP1 (high-molecular-weight protein 1) and HMWP2 (high-molecular-weight protein 2), and hydrolyzes a variety of aromatic and acyl groups from their phosphopantetheinylated carrier protein domains. YbtT titration in uropathogenic revealed a distinct optimum for Ybt production consistent with a tradeoff between clearing both stalled inhibitory intermediates and productive Ybt precursors from HMWP1 and HMWP2. These results are consistent with a model in which YbtT maintains cellular Ybt biosynthesis by removing nonproductive, inhibitory thioesters that form aberrantly at multiple sites on HMWP1 and HMWP2.

PubMed: 30355735
DOI: 10.1074/jbc.RA118.005752

実験手法

X-RAY DIFFRACTION (1.9 Å)