6B7K

GH43 Endo-Arabinanase from Bacillus licheniformis

6B7K の概要

• エントリーDOI: 10.2210/pdb6b7k/pdb
• 分子名称: Endo-alpha-(1->5)-L-arabinanase, CALCIUM ION (3 entities in total)
• 機能のキーワード: arabinanase, bacillus licheniformis, gh43, hydrolase
• 由来する生物種: Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 134320.56

構造登録者

Farro, E.G.S.,Nascimento, A.S. (登録日: 2017-10-04, 公開日: 2018-06-13, 最終更新日: 2023-10-04)

主引用文献

Farro, E.G.S.,Leite, A.E.T.,Silva, I.A.,Filgueiras, J.G.,de Azevedo, E.R.,Polikarpov, I.,Nascimento, A.S.
GH43 endo-arabinanase from Bacillus licheniformis: Structure, activity and unexpected synergistic effect on cellulose enzymatic hydrolysis.
Int. J. Biol. Macromol., 117:7-16, 2018

PubMed Abstract: The hydrolysis of the plant biomass provides many interesting opportunities for the generation of building blocks for the green chemistry industrial applications. An important progress has been made for the hydrolysis of the cellulosic component of the biomass while, for the hemicellulosic components, the advances are less straightforward. Here, we describe the cloning, expression and biochemical and structural characterization of BlAbn1, a GH43 arabinanase from Bacillus licheniformis. This enzyme is selective for linear arabinan and efficiently hydrolyzes this substrate, with a specific activity of 127 U/mg. The enzyme has optimal conditions for activity at pH 8.0 and 45 °C and its activity is only partially dependent of a bound calcium ion since 70% of the maximal activity is preserved even when 1 mM EDTA is added to the reaction medium. BlAbn1 crystal structure revealed a typical GH43 fold and narrow active site, which explains the selectivity for linear substrates. Unexpectedly, the enzyme showed a synergic effect with the commercial cocktail Accellerase 1500 on cellulose hydrolysis. Scanning Electron Microscopy, Solid-State NMR and relaxometry data indicate that the enzyme weakens the interaction between cellulose fibers in filter paper, thus providing an increased access to the cellulases of the cocktail.

PubMed: 29800670
DOI: 10.1016/j.ijbiomac.2018.05.157

実験手法

X-RAY DIFFRACTION (2.55 Å)