6B4V

Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome

これはPDB形式変換不可エントリーです。

6B4V の概要

• エントリーDOI: 10.2210/pdb6b4v/pdb
• 分子名称: 16S ribosomal RNA, 50S ribosomal protein L9, 50S ribosomal protein L13, ... (58 entities in total)
• 機能のキーワード: termination suppression, class i release factors, rf1, stop-codon recognition, termination, blasticidin s, ribosome-antibiotic complex, ribosome/antibiotic
• 由来する生物種: Thermus thermophilus HB27; 詳細
• タンパク質・核酸の鎖数: 112
• 化学式量合計: 4544266.41

構造登録者

Svidritskiy, E.,Korostelev, A.A. (登録日: 2017-09-27, 公開日: 2018-02-07, 最終更新日: 2025-03-19)

主引用文献

Svidritskiy, E.,Korostelev, A.A.
Mechanism of Inhibition of Translation Termination by Blasticidin S.
J. Mol. Biol., 430:591-593, 2018

PubMed Abstract: Understanding the mechanisms of inhibitors of translation termination may inform development of new antibacterials and therapeutics for premature termination diseases. We report the crystal structure of the potent termination inhibitor blasticidin S bound to the ribosomal 70S•release factor 1 (RF1) termination complex. Blasticidin S shifts the catalytic domain 3 of RF1 and restructures the peptidyl transferase center. Universally conserved uridine 2585 in the peptidyl transferase center occludes the catalytic backbone of the GGQ motif of RF1, explaining the structural mechanism of inhibition. Rearrangement of domain 3 relative to the codon-recognition domain 2 provides insight into the dynamics of RF1 implicated in termination accuracy.

PubMed: 29366636
DOI: 10.1016/j.jmb.2018.01.007

実験手法

X-RAY DIFFRACTION (3.4 Å)