6AZC

Crystal structure of Physcomitrella patens KAI2-like E S166A

6AZC の概要

• エントリーDOI: 10.2210/pdb6azc/pdb
• 分子名称: Pp-KAI2-like E (2 entities in total)
• 機能のキーワード: alpha/beta hydrolase, strigolactone binding, hydrolase
• 由来する生物種: Physcomitrella patens subsp. patens (Moss)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 59758.23

構造登録者

Burger, M.,Lee, H.J.,Chory, J. (登録日: 2017-09-11, 公開日: 2019-02-06, 最終更新日: 2023-10-04)

主引用文献

Burger, M.,Mashiguchi, K.,Lee, H.J.,Nakano, M.,Takemoto, K.,Seto, Y.,Yamaguchi, S.,Chory, J.
Structural Basis of Karrikin and Non-natural Strigolactone Perception in Physcomitrella patens.
Cell Rep, 26:855-865.e5, 2019

PubMed Abstract: In plants, strigolactones are perceived by the dual receptor-hydrolase DWARF14 (D14). D14 belongs to the superfamily of α/β hydrolases and is structurally similar to the karrikin receptor KARRIKIN INSENSITIVE 2 (KAI2). The moss Physcomitrella patens is an ideal model system for studying this receptor family, because it includes 11 highly related family members with unknown ligand specificity. We present the crystal structures of three Physcomitrella D14/KAI2-like proteins and describe a loop-based mechanism that leads to a permanent widening of the hydrophobic substrate gorge. We have identified protein clades that specifically perceive the karrikin KAR and the non-natural strigolactone isomer (-)-5-deoxystrigol in a highly stereoselective manner.

PubMed: 30673608
DOI: 10.1016/j.celrep.2019.01.003

実験手法

X-RAY DIFFRACTION (2.00001213332 Å)