6AXZ

Segment from bank vole prion protein 168-176 QYNNQNNFV

Summary for 6AXZ

• Entry DOI: 10.2210/pdb6axz/pdb
• EMDB information: 7017
• Descriptor: Major prion protein (2 entities in total)
• Functional Keywords: polar clasp, amyloid fibril, prion, protein fibril
• Biological source: Myodes glareolus (Bank vole)
• Cellular location: Cell membrane ; Lipid-anchor, GPI-anchor : Q8VHV5
• Total number of polymer chains: 1
• Total formula weight: 1140.16

Authors

Glynn, C.,Rodriguez, J.A.,Boyer, D.R.,Gallagher-Jones, M. (deposition date: 2017-09-07, release date: 2018-01-17, Last modification date: 2024-03-13)

Primary citation

Gallagher-Jones, M.,Glynn, C.,Boyer, D.R.,Martynowycz, M.W.,Hernandez, E.,Miao, J.,Zee, C.T.,Novikova, I.V.,Goldschmidt, L.,McFarlane, H.T.,Helguera, G.F.,Evans, J.E.,Sawaya, M.R.,Cascio, D.,Eisenberg, D.S.,Gonen, T.,Rodriguez, J.A.
Sub-angstrom cryo-EM structure of a prion protofibril reveals a polar clasp.
Nat. Struct. Mol. Biol., 25:131-134, 2018

PubMed Abstract: The atomic structure of the infectious, protease-resistant, β-sheet-rich and fibrillar mammalian prion remains unknown. Through the cryo-EM method MicroED, we reveal the sub-ångström-resolution structure of a protofibril formed by a wild-type segment from the β2-α2 loop of the bank vole prion protein. The structure of this protofibril reveals a stabilizing network of hydrogen bonds that link polar zippers within a sheet, producing motifs we have named 'polar clasps'.

PubMed: 29335561
DOI: 10.1038/s41594-017-0018-0

Experimental method

ELECTRON CRYSTALLOGRAPHY (0.75 Å)