6AXZ

Segment from bank vole prion protein 168-176 QYNNQNNFV

6AXZ の概要

• エントリーDOI: 10.2210/pdb6axz/pdb
• EMDBエントリー: 7017
• 分子名称: Major prion protein (2 entities in total)
• 機能のキーワード: polar clasp, amyloid fibril, prion, protein fibril
• 由来する生物種: Myodes glareolus (Bank vole)
• 細胞内の位置: Cell membrane ; Lipid-anchor, GPI-anchor : Q8VHV5
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 1140.16

構造登録者

Glynn, C.,Rodriguez, J.A.,Boyer, D.R.,Gallagher-Jones, M. (登録日: 2017-09-07, 公開日: 2018-01-17, 最終更新日: 2024-03-13)

主引用文献

Gallagher-Jones, M.,Glynn, C.,Boyer, D.R.,Martynowycz, M.W.,Hernandez, E.,Miao, J.,Zee, C.T.,Novikova, I.V.,Goldschmidt, L.,McFarlane, H.T.,Helguera, G.F.,Evans, J.E.,Sawaya, M.R.,Cascio, D.,Eisenberg, D.S.,Gonen, T.,Rodriguez, J.A.
Sub-angstrom cryo-EM structure of a prion protofibril reveals a polar clasp.
Nat. Struct. Mol. Biol., 25:131-134, 2018

PubMed Abstract: The atomic structure of the infectious, protease-resistant, β-sheet-rich and fibrillar mammalian prion remains unknown. Through the cryo-EM method MicroED, we reveal the sub-ångström-resolution structure of a protofibril formed by a wild-type segment from the β2-α2 loop of the bank vole prion protein. The structure of this protofibril reveals a stabilizing network of hydrogen bonds that link polar zippers within a sheet, producing motifs we have named 'polar clasps'.

PubMed: 29335561
DOI: 10.1038/s41594-017-0018-0

実験手法

ELECTRON CRYSTALLOGRAPHY (0.75 Å)