6AV7

Structure of human endothelial nitric oxide synthase heme domain in complex with HW69

6AV7 の概要

• エントリーDOI: 10.2210/pdb6av7/pdb
• 関連するPDBエントリー: 6AUQ 6AUR 6AUS 6AUT 6AUU 6AUV 6AUW 6AUX 6AUY 6AUZ 6AV0 6AV1 6AV2 6AV3 6AV4 6AV5 6AV6
• 分子名称: Nitric oxide synthase, endothelial, PROTOPORPHYRIN IX CONTAINING FE, 5,6,7,8-TETRAHYDROBIOPTERIN, ... (10 entities in total)
• 機能のキーワード: nitric oxide synthase inhibitor complex heme enzyme, oxidoreductase
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 205044.71

構造登録者

Li, H.,Poulos, T.L. (登録日: 2017-09-01, 公開日: 2018-07-11, 最終更新日: 2023-10-04)

主引用文献

Do, H.T.,Wang, H.Y.,Li, H.,Chreifi, G.,Poulos, T.L.,Silverman, R.B.
Improvement of Cell Permeability of Human Neuronal Nitric Oxide Synthase Inhibitors Using Potent and Selective 2-Aminopyridine-Based Scaffolds with a Fluorobenzene Linker.
J. Med. Chem., 60:9360-9375, 2017

PubMed Abstract: Inhibition of neuronal nitric oxide synthase (nNOS) is a promising therapeutic approach to treat neurodegenerative diseases. Recently, we have achieved considerable progress in improving the potency and isoform selectivity of human nNOS inhibitors bearing a 2-aminopyridine scaffold. However, these inhibitors still suffered from too low cell membrane permeability to enter into CNS drug development. We report herein our studies to improve permeability of nNOS inhibitors as measured by both PAMPA-BBB and Caco-2 assays. The most permeable compound (12) in this study still preserves excellent potency with human nNOS (K = 30 nM) and very high selectivity over other NOS isoforms, especially human eNOS (hnNOS/heNOS = 2799, the highest hnNOS/heNOS ratio we have obtained to date). X-ray crystallographic analysis reveals that 12 adopts a similar binding mode in both rat and human nNOS, in which the 2-aminopyridine and the fluorobenzene linker form crucial hydrogen bonds with glutamate and tyrosine residues, respectively.

PubMed: 29091437
DOI: 10.1021/acs.jmedchem.7b01356

実験手法

X-RAY DIFFRACTION (1.916 Å)