6ASX

CryoEM structure of E.coli his pause elongation complex

6ASX の概要

• エントリーDOI: 10.2210/pdb6asx/pdb
• EMDBエントリー: 7002
• 分子名称: DNA (32-MER), RNA (29-MER), DNA-directed RNA polymerase subunit alpha, ... (9 entities in total)
• 機能のキーワード: dna-dependent rna polymerase, transcription, transcription-dna-rna complex, transcription/dna/rna
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 397573.77

構造登録者

Kang, J.Y.,Landick, R.,Darst, S.A. (登録日: 2017-08-25, 公開日: 2018-03-28, 最終更新日: 2024-03-13)

主引用文献

Kang, J.Y.,Mishanina, T.V.,Bellecourt, M.J.,Mooney, R.A.,Darst, S.A.,Landick, R.
RNA Polymerase Accommodates a Pause RNA Hairpin by Global Conformational Rearrangements that Prolong Pausing.
Mol. Cell, 69:802-815.e1, 2018

PubMed Abstract: Sequence-specific pausing by RNA polymerase (RNAP) during transcription plays crucial and diverse roles in gene expression. In bacteria, RNA structures are thought to fold within the RNA exit channel of the RNAP and can increase pause lifetimes significantly. The biophysical mechanism of pausing is uncertain. We used single-particle cryo-EM to determine structures of paused complexes, including a 3.8-Å structure of an RNA hairpin-stabilized, paused RNAP that coordinates RNA folding in the his operon attenuation control region of E. coli. The structures revealed a half-translocated pause state (RNA post-translocated, DNA pre-translocated) that can explain transcriptional pausing and a global conformational change of RNAP that allosterically inhibits trigger loop folding and can explain pause hairpin action. Pause hairpin interactions with the RNAP RNA exit channel suggest how RNAP guides the formation of nascent RNA structures.

PubMed: 29499135
DOI: 10.1016/j.molcel.2018.01.018

実験手法

ELECTRON MICROSCOPY (3.8 Å)