6ANO
Crystal structure of human FLASH N-terminal domain
6ANO の概要
エントリーDOI | 10.2210/pdb6ano/pdb |
分子名称 | CASP8-associated protein 2 (2 entities in total) |
機能のキーワード | coiled-coil, gene regulation |
由来する生物種 | Homo sapiens (Human) |
細胞内の位置 | Cytoplasm: Q9UKL3 |
タンパク質・核酸の鎖数 | 2 |
化学式量合計 | 23045.17 |
構造登録者 | |
主引用文献 | Aik, W.S.,Lin, M.H.,Tan, D.,Tripathy, A.,Marzluff, W.F.,Dominski, Z.,Chou, C.Y.,Tong, L. The N-terminal domains of FLASH and Lsm11 form a 2:1 heterotrimer for histone pre-mRNA 3'-end processing. PLoS ONE, 12:e0186034-e0186034, 2017 Cited by PubMed Abstract: Unlike canonical pre-mRNAs, animal replication-dependent histone pre-mRNAs lack introns and are processed at the 3'-end by a mechanism distinct from cleavage and polyadenylation. They have a 3' stem loop and histone downstream element (HDE) that are recognized by stem-loop binding protein (SLBP) and U7 snRNP, respectively. The N-terminal domain (NTD) of Lsm11, a component of U7 snRNP, interacts with FLASH NTD and these two proteins recruit the histone cleavage complex containing the CPSF-73 endonuclease for the cleavage reaction. Here, we determined crystal structures of FLASH NTD and found that it forms a coiled-coil dimer. Using solution light scattering, we characterized the stoichiometry of the FLASH NTD-Lsm11 NTD complex and found that it is a 2:1 heterotrimer, which is supported by observations from analytical ultracentrifugation and crosslinking. PubMed: 29020104DOI: 10.1371/journal.pone.0186034 主引用文献が同じPDBエントリー |
実験手法 | X-RAY DIFFRACTION (2.61 Å) |
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