6AND

Pinatuzumab Fab in complex with anti-Kappa VHH domain

Summary for 6AND

• Entry DOI: 10.2210/pdb6and/pdb
• Descriptor: Anti-kappa VHH domain, Pinatuzumab Fab Heavy chain, Pinatuzumab Fab light chain, ... (5 entities in total)
• Functional Keywords: fab, antibody, cd22, vhh domain, immune system
• Biological source: Lama glama; More
• Total number of polymer chains: 3
• Total formula weight: 58304.06

Authors

Ereno-Orbea, J.,Sicard, T.,Julien, J.-P. (deposition date: 2017-08-12, release date: 2018-01-31, Last modification date: 2024-11-13)

Primary citation

Ereno-Orbea, J.,Sicard, T.,Cui, H.,Carson, J.,Hermans, P.,Julien, J.P.
Structural Basis of Enhanced Crystallizability Induced by a Molecular Chaperone for Antibody Antigen-Binding Fragments.
J. Mol. Biol., 430:322-336, 2018

PubMed Abstract: Monoclonal antibodies constitute one of the largest groups of drugs to treat cancers and immune disorders, and are guiding the design of vaccines against infectious diseases. Fragments antigen-binding (Fabs) have been preferred over monoclonal antibodies for the structural characterization of antibody-antigen complexes due to their relatively low flexibility. Nonetheless, Fabs often remain challenging to crystallize because of the surface characteristics of complementary determining regions and the residual flexibility in the hinge region between the variable and constant domains. Here, we used a variable heavy-chain (VH) domain specific for the human kappa light chain to assist in the structure determination of three therapeutic Fabs that were recalcitrant to crystallization on their own. We show that this ligand alters the surface properties of the antibody-ligand complex and lowers its aggregation temperature to favor crystallization. The VH crystallization chaperone also restricts the flexible hinge of Fabs to a narrow range of angles, and so independently of the variable region. Our findings contribute a valuable approach to antibody structure determination and provide biophysical insight into the principles that govern the crystallization of macromolecules.

PubMed: 29277294
DOI: 10.1016/j.jmb.2017.12.010

Experimental method

X-RAY DIFFRACTION (1.75 Å)