6AL2
Crystal structure of E. coli YidC at 2.8 A resolution
Summary for 6AL2
| Entry DOI | 10.2210/pdb6al2/pdb |
| Descriptor | Membrane protein insertase YidC, (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate (3 entities in total) |
| Functional Keywords | transmembrane, insertase, chaperone |
| Biological source | Escherichia coli |
| Total number of polymer chains | 2 |
| Total formula weight | 124101.95 |
| Authors | Tanaka, Y.,Tsukazaki, T.,Izumioka, A.,Hamid, A.A.,Fujii, A. (deposition date: 2018-09-05, release date: 2018-11-07, Last modification date: 2023-11-22) |
| Primary citation | Tanaka, Y.,Izumioka, A.,Abdul Hamid, A.,Fujii, A.,Haruyama, T.,Furukawa, A.,Tsukazaki, T. 2.8-angstrom crystal structure of Escherichia coli YidC revealing all core regions, including flexible C2 loop. Biochem. Biophys. Res. Commun., 505:141-145, 2018 Cited by PubMed Abstract: YidC/Alb3/Oxa1 family proteins are involved in the insertion and assembly of membrane proteins. The core five transmembrane regions of YidC, which are conserved in the protein family, form a positively charged cavity open to the cytoplasmic side. The cavity plays an important role in membrane protein insertion. In all reported structural studies of YidC, the second cytoplasmic loop (C2 loop) was disordered, limiting the understanding of its role. Here, we determined the crystal structure of YidC including the C2 loop at 2.8 Å resolution with R/R = 21.8/27.5. This structure and subsequent molecular dynamics simulation indicated that the intrinsic flexible C2 loop covered the positively charged cavity. This crystal structure provides the coordinates of the complete core region including the C2 loop, which is valuable for further analyses of YidC. PubMed: 30241934DOI: 10.1016/j.bbrc.2018.09.043 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.8 Å) |
Structure validation
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