6AL2

Crystal structure of E. coli YidC at 2.8 A resolution

6AL2 の概要

• エントリーDOI: 10.2210/pdb6al2/pdb
• 分子名称: Membrane protein insertase YidC, (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate (3 entities in total)
• 機能のキーワード: transmembrane, insertase, chaperone
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 124101.95

構造登録者

Tanaka, Y.,Tsukazaki, T.,Izumioka, A.,Hamid, A.A.,Fujii, A. (登録日: 2018-09-05, 公開日: 2018-11-07, 最終更新日: 2023-11-22)

主引用文献

Tanaka, Y.,Izumioka, A.,Abdul Hamid, A.,Fujii, A.,Haruyama, T.,Furukawa, A.,Tsukazaki, T.
2.8-angstrom crystal structure of Escherichia coli YidC revealing all core regions, including flexible C2 loop.
Biochem. Biophys. Res. Commun., 505:141-145, 2018

PubMed Abstract: YidC/Alb3/Oxa1 family proteins are involved in the insertion and assembly of membrane proteins. The core five transmembrane regions of YidC, which are conserved in the protein family, form a positively charged cavity open to the cytoplasmic side. The cavity plays an important role in membrane protein insertion. In all reported structural studies of YidC, the second cytoplasmic loop (C2 loop) was disordered, limiting the understanding of its role. Here, we determined the crystal structure of YidC including the C2 loop at 2.8 Å resolution with R/R = 21.8/27.5. This structure and subsequent molecular dynamics simulation indicated that the intrinsic flexible C2 loop covered the positively charged cavity. This crystal structure provides the coordinates of the complete core region including the C2 loop, which is valuable for further analyses of YidC.

PubMed: 30241934
DOI: 10.1016/j.bbrc.2018.09.043

実験手法

X-RAY DIFFRACTION (2.8 Å)