6AKL

Crystal structure of Striatin3 in complex with SIKE1 Coiled-coil domain

Summary for 6AKL

• Entry DOI: 10.2210/pdb6akl/pdb
• Descriptor: Suppressor of IKBKE 1, Striatin-3 (3 entities in total)
• Functional Keywords: coiled-coil domain, heterotrimer, protein binding
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 3
• Total formula weight: 16034.38

Authors

Zhou, L.,Chen, M.,Zhou, Z.C. (deposition date: 2018-09-02, release date: 2019-01-16, Last modification date: 2023-11-22)

Primary citation

Tang, Y.,Chen, M.,Zhou, L.,Ma, J.,Li, Y.,Zhang, H.,Shi, Z.,Xu, Q.,Zhang, X.,Gao, Z.,Zhao, Y.,Cheng, Y.,Jiao, S.,Zhou, Z.
Architecture, substructures, and dynamic assembly of STRIPAK complexes in Hippo signaling.
Cell Discov, 5:3-3, 2019

PubMed Abstract: Striatin-interacting phosphatases and kinases (STRIPAKs) are evolutionarily conserved supramolecular complexes, which have been implicated in the Hippo signaling pathway. Yet the topological structure and dynamic assembly of STRIPAK complexes remain elusive. Here, we report the overall architecture and substructures of a Hippo kinase-containing STRIPAK complex. PP2Aa/c-bound STRN3 directly contacts the Hippo kinase MST2 and also controls the loading of MST2 via two "arms" in a phosphorylation-dependent manner, one arm being STRIP1 and the other SIKE1-SLMAP. A decreased cell density triggered the dissociation of the STRIP1 arm from STRIPAK, reflecting the dynamic assembly of the complex upon sensing upstream signals. Crystallographic studies defined at atomic resolution the interface between STRN3 and SIKE1, and that between SIKE1 and SLMAP. Disrupting the complex assembly abrogated the regulatory effect of STRIPAK towards Hippo signaling. Collectively, our study revealed a "two-arm" assembly of STRIPAK with context-dependent dynamics, offering a framework for further studies on Hippo signaling and biological processes involving MST kinases.

PubMed: 30622739
DOI: 10.1038/s41421-018-0077-3

Experimental method

X-RAY DIFFRACTION (1.75 Å)