6AKE

Crystal structure of mouse claudin-3 in complex with C-terminal fragment of Clostridium perfringens enterotoxin

6AKE の概要

• エントリーDOI: 10.2210/pdb6ake/pdb
• 分子名称: Claudin-3, Heat-labile enterotoxin B chain (2 entities in total)
• 機能のキーワード: cell adhesion, tight junction, membrane protein-toxin complex, membrane protein/toxin
• 由来する生物種: Mus musculus (Mouse); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 66472.67

構造登録者

Nakamura, S.,Irie, K.,Fujiyoshi, Y. (登録日: 2018-08-31, 公開日: 2019-02-27, 最終更新日: 2024-10-23)

主引用文献

Nakamura, S.,Irie, K.,Tanaka, H.,Nishikawa, K.,Suzuki, H.,Saitoh, Y.,Tamura, A.,Tsukita, S.,Fujiyoshi, Y.
Morphologic determinant of tight junctions revealed by claudin-3 structures.
Nat Commun, 10:816-816, 2019

PubMed Abstract: Tight junction is a cell adhesion apparatus functioning as barrier and/or channel in the paracellular spaces of epithelia. Claudin is the major component of tight junction and polymerizes to form tight junction strands with various morphologies that may correlate with their functions. Here we present the crystal structure of mammalian claudin-3 at 3.6 Å resolution. The third transmembrane helix of claudin-3 is clearly bent compared with that of other subtypes. Structural analysis of additional two mutants with a single mutation representing other subtypes in the third helix indicates that this helix takes a bent or straight structure depending on the residue. The presence or absence of the helix bending changes the positions of residues related to claudin-claudin interactions and affects the morphology and adhesiveness of the tight junction strands. These results evoke a model for tight junction strand formation with different morphologies - straight or curvy strands - observed in native epithelia.

PubMed: 30778075
DOI: 10.1038/s41467-019-08760-7

実験手法

X-RAY DIFFRACTION (3.6 Å)