6AHF

CryoEM Reconstruction of Hsp104 N728A Hexamer

Summary for 6AHF

• Entry DOI: 10.2210/pdb6ahf/pdb
• EMDB information: 9625
• Descriptor: Heat shock protein 104, PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER (2 entities in total)
• Functional Keywords: chaperone, hsp, holdase
• Biological source: Saccharomyces cerevisiae S288c (Baker's yeast)
• Total number of polymer chains: 6
• Total formula weight: 615925.11

Authors

Zhang, X.,Zhang, L.,Zhang, S. (deposition date: 2018-08-17, release date: 2019-02-13, Last modification date: 2025-07-02)

Primary citation

Zhang, X.,Zhang, S.,Zhang, L.,Lu, J.,Zhao, C.,Luo, F.,Li, D.,Li, X.,Liu, C.
Heat shock protein 104 (HSP104) chaperones soluble Tau via a mechanism distinct from its disaggregase activity.
J. Biol. Chem., 294:4956-4965, 2019

PubMed Abstract: Heat shock protein 104 (HSP104) is a conserved AAA+ protein disaggregase, can disassemble the toxic aggregates formed by different amyloid proteins, and is protective in various animal models associated with amyloid-related diseases. Extensive studies have attempted to elucidate how HSP104 disassembles the aggregated form of clients. Here, we found that HSP104 exhibits a potent holdase activity that does not require energy, prevents the soluble form of amyloid clients from aggregating, and differs from HSP104's disaggregase activity. Using cryo-EM, NMR, and additional biophysical approaches, we found that HSP104 utilizes its small subdomain of nucleotide-binding domain 2 (ssNBD2) to capture the soluble amyloid client (K19 of Tau) independent of its ATP hydrolysis activity. Our results indicate that HSP104 utilizes two fundamental distinct mechanisms to chaperone different forms of amyloid client and highlight the important yet previously unappreciated function of ssNBD2 in chaperoning amyloid client and thereby preventing pathological aggregation.

PubMed: 30718279
DOI: 10.1074/jbc.RA118.005980

Experimental method

ELECTRON MICROSCOPY (6.78 Å)