6ABY
Crystal structure of citrate synthase (Msed_1522) from Metallosphaera sedula in complex with oxaloacetate
Summary for 6ABY
| Entry DOI | 10.2210/pdb6aby/pdb |
| Descriptor | Citrate synthase, OXALOACETATE ION, GLYCEROL, ... (5 entities in total) |
| Functional Keywords | citrate synthase, metallosphaera sedula, transferase |
| Biological source | Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2) |
| Total number of polymer chains | 2 |
| Total formula weight | 88526.52 |
| Authors | Lee, S.-H.,Son, H.-F.,Kim, K.-J. (deposition date: 2018-07-24, release date: 2019-03-20, Last modification date: 2023-11-22) |
| Primary citation | Lee, S.H.,Son, H.F.,Kim, K.J. Structural insights into the inhibition properties of archaeon citrate synthase from Metallosphaera sedula. PLoS ONE, 14:e0212807-e0212807, 2019 Cited by PubMed Abstract: Metallosphaera sedula is a thermoacidophilic archaeon and has an incomplete TCA/glyoxylate cycle that is used for production of biosynthetic precursors of essential metabolites. Citrate synthase from M. sedula (MsCS) is an enzyme involved in the first step of the incomplete TCA/glyoxylate cycle by converting oxaloacetate and acetyl-CoA into citrate and coenzyme A. To elucidate the inhibition properties of MsCS, we determined its crystal structure at 1.7 Å resolution. Like other Type-I CS, MsCS functions as a dimer and each monomer consists of two distinct domains, a large domain and a small domain. The oxaloacetate binding site locates at the cleft between the two domains, and the active site was more closed upon binding of the oxaloacetate substrate than binding of the citrate product. Interestingly, the inhibition kinetic analysis showed that, unlike other Type-I CSs, MsCS is non-competitively inhibited by NADH. Finally, amino acids and structural comparison of MsCS with other Type-II CSs, which were reported to be non-competitively inhibited by NADH, revealed that MsCS has quite unique NADH binding mode for non-competitive inhibition. PubMed: 30794680DOI: 10.1371/journal.pone.0212807 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
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