6AB8

Crystal structure of Methanosarcina mazei PylRS(Y306A/Y384F) complexed with ZLys

Summary for 6AB8

Related6AAC 6AAD 6AAN 6AAO 6AAP 6AAQ 6AAZ 6AB0 6AB1 6AB2
DescriptorPyrrolysine--tRNA ligase, MAGNESIUM ION, ADENOSINE-5'-TRIPHOSPHATE, ... (6 entities in total)
Functional Keywordsaminoacyl-trna synthetase, pyrrolysyl-trna synthetase, trna, non-natural amino acids, translation
Biological sourceMethanosarcina mazei JCM 9314 (Methanosarcina frisia)
Total number of polymer chains1
Total molecular weight32565.91
Authors
Yanagisawa, T.,Kuratani, M.,Yokoyama, S. (deposition date: 2018-07-20, release date: 2019-04-17, Last modification date: 2019-05-22)
Primary citation
Yanagisawa, T.,Kuratani, M.,Seki, E.,Hino, N.,Sakamoto, K.,Yokoyama, S.
Structural Basis for Genetic-Code Expansion with Bulky Lysine Derivatives by an Engineered Pyrrolysyl-tRNA Synthetase.
Cell Chem Biol, 2019
PubMed: 31031143 (PDB entries with the same primary citation)
DOI: 10.1016/j.chembiol.2019.03.008
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.753 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.21340 1.3% 4.2%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 6ab8
no rotation
Molmil generated image of 6ab8
rotated about x axis by 90°
Molmil generated image of 6ab8
rotated about y axis by 90°

More Biological unit images

Molmil generated image of 6ab8
no rotation
Molmil generated image of 6ab8
rotated about x axis by 90°
Molmil generated image of 6ab8
rotated about y axis by 90°
(*)In the case of coarse surface representation, the asymmetric unit is shown as red ribbon representation.
Coordinate files for Biological unit (6ab8.pdb1.gz [100.91 KB])