6AA7

Fluorescent protein from Acropora digitifera

6AA7 の概要

• エントリーDOI: 10.2210/pdb6aa7/pdb
• 分子名称: Fluorescent protein (2 entities in total)
• 機能のキーワード: fluorescent protein, red fluorescent protein, acropora digitifera, chromophore
• 由来する生物種: Acropora digitifera (Staghorn coral)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 52840.48

構造登録者

Kim, S.E.,Hwang, K.Y.,Nam, K.H. (登録日: 2018-07-17, 公開日: 2018-08-22, 最終更新日: 2024-10-09)

主引用文献

Kim, S.E.,Hwang, K.Y.,Nam, K.H.
Spectral and structural analysis of a red fluorescent protein from Acropora digitifera.
Protein Sci., 28:375-381, 2019

PubMed Abstract: Fluorescent proteins (FPs) possess a wide variety of spectral properties that make them of widespread interest as optical markers. These proteins can be applied as pH indicators or metal biosensors. The discovery and characterization of new fluorescent proteins is expected to further extend their application. Here, we report the spectral and structural analysis of a red fluorescent protein from Acropora digitifera (designated AdRed). This protein shows a tetrameric state and is red emitting, with excitation and emission maxima at 567 and 612 nm, respectively. Its crystal structure shows the tetrameric interface stabilized by hydrogen bonding and salt bridges. The electron density map of the chromophore, consisting of Asp66-Tyr67-Gly68, shows the decarboxylated side chain of Asp66. Ser223, located near the chromophore, has the role of bridging His202 and Glu221, and is part of the hydrogen bond network. Mutated AdRed with Cys148Ser reveals a blue shift in fluorescence excitation and emission. Our results provide insights into understanding the molecular function of AdRed and other FPs.

PubMed: 30368951
DOI: 10.1002/pro.3540

実験手法

X-RAY DIFFRACTION (1.8 Å)