6A9X

Crystal Structure of AnkG/GABARAP Complex

Summary for 6A9X

• Entry DOI: 10.2210/pdb6a9x/pdb
• Descriptor: Gamma-aminobutyric acid receptor-associated protein, Ankyrin-3 (3 entities in total)
• Functional Keywords: protein binding, structural protein, signaling protein
• Biological source: Mus musculus (Mouse); More
• Total number of polymer chains: 2
• Total formula weight: 16635.82

Authors

Wang, C.,Li, J.,Chen, K.,Zhang, M. (deposition date: 2018-07-16, release date: 2018-12-26, Last modification date: 2023-11-22)

Primary citation

Nelson, A.D.,Caballero-Floran, R.N.,Rodriguez Diaz, J.C.,Hull, J.M.,Yuan, Y.,Li, J.,Chen, K.,Walder, K.K.,Lopez-Santiago, L.F.,Bennett, V.,McInnis, M.G.,Isom, L.L.,Wang, C.,Zhang, M.,Jones, K.S.,Jenkins, P.M.
Ankyrin-G regulates forebrain connectivity and network synchronization via interaction with GABARAP.
Mol. Psychiatry, 2018

PubMed Abstract: GABAergic circuits are critical for the synchronization and higher order function of brain networks. Defects in this circuitry are linked to neuropsychiatric diseases, including bipolar disorder, schizophrenia, and autism. Work in cultured neurons has shown that ankyrin-G plays a key role in the regulation of GABAergic synapses on the axon initial segment and somatodendritic domain of pyramidal neurons, where it interacts directly with the GABA receptor-associated protein (GABARAP) to stabilize cell surface GABA receptors. Here, we generated a knock-in mouse model expressing a mutation that abolishes the ankyrin-G/GABARAP interaction (Ank3 W1989R) to understand how ankyrin-G and GABARAP regulate GABAergic circuitry in vivo. We found that Ank3 W1989R mice exhibit a striking reduction in forebrain GABAergic synapses resulting in pyramidal cell hyperexcitability and disruptions in network synchronization. In addition, we identified changes in pyramidal cell dendritic spines and axon initial segments consistent with compensation for hyperexcitability. Finally, we identified the ANK3 W1989R variant in a family with bipolar disorder, suggesting a potential role of this variant in disease. Our results highlight the importance of ankyrin-G in regulating forebrain circuitry and provide novel insights into how ANK3 loss-of-function variants may contribute to human disease.

PubMed: 30504823
DOI: 10.1038/s41380-018-0308-x

Experimental method

X-RAY DIFFRACTION (2.202 Å)