6A9T

Crystal structure of Icp55 from Saccharomyces cerevisiae (N-terminal 58 residues deletion)

6A9T の概要

• エントリーDOI: 10.2210/pdb6a9t/pdb
• 分子名称: Intermediate cleaving peptidase 55, MANGANESE (II) ION, GLYCINE, ... (5 entities in total)
• 機能のキーワード: intermediate cleaving peptidase 55, m24b, peptidase, xaa-pro aminopeptidase, mitochondrial, hydrolase
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 52141.46

構造登録者

Singh, R.,Kumar, A.,Goyal, V.D.,Makde, R.D. (登録日: 2018-07-16, 公開日: 2019-01-16, 最終更新日: 2023-11-22)

主引用文献

Singh, R.,Goyal, V.D.,Kumar, A.,Sabharwal, N.S.,Makde, R.D.
Crystal structures and biochemical analyses of intermediate cleavage peptidase: role of dynamics in enzymatic function.
FEBS Lett., 593:443-454, 2019

PubMed Abstract: Intermediate cleavage peptidase (Icp55) processes a subset of mitochondrial matrix proteins by removing a bulky residue at their N termini, leaving behind smaller N-terminal residues (icp activity). This contributes towards the stability of the mitochondrial proteome. We report crystal structures of yeast Icp55 including one bound to the apstatin inhibitor. Apart from icp activity, the enzyme was found to exhibit Xaa-Pro aminopeptidase activity in vitro. Structural and biochemical data suggest that the enzyme exists in a rapid equilibrium between monomer and dimer. Furthermore, the dimer, and not the monomer, was found to be the active species with loop dynamics at the dimer interface playing an important role in activity. Based on the new evidence, we propose a model for binding and processing of cellular targets by Icp55. DATABASE: The atomic coordinates and structure factors for the structures of Icp55 (code 6A9T, 6A9U, 6A9V) have been deposited in the Protein Data Bank (PDB) (http://www.pdb.org/).

PubMed: 30582634
DOI: 10.1002/1873-3468.13321

実験手法

X-RAY DIFFRACTION (2.15 Å)