6A8H

Crystal structure of endo-arabinanase ABN-TS D27A mutant in complex with arabinotriose

6A8H の概要

• エントリーDOI: 10.2210/pdb6a8h/pdb
• 分子名称: endo-alpha-(1->5)-L-arabinanase, alpha-L-arabinofuranose-(1-5)-alpha-L-arabinofuranose-(1-5)-alpha-L-arabinofuranose, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: arabinanase, thermostable enzyme, glycoside hydrolase, gh43, hydrolase
• 由来する生物種: Geobacillus thermodenitrificans
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37407.82

構造登録者

Yamaguchi, A.,Tada, T. (登録日: 2018-07-09, 公開日: 2018-12-19, 最終更新日: 2024-11-06)

主引用文献

Yamaguchi, A.,Sogabe, Y.,Fukuoka, S.,Sakai, T.,Tada, T.
Structures of endo-1,5-alpha-L-arabinanase mutants from Bacillus thermodenitrificans TS-3 in complex with arabino-oligosaccharides.
Acta Crystallogr F Struct Biol Commun, 74:774-780, 2018

PubMed Abstract: The thermostable endo-1,5-α-L-arabinanase from Bacillus thermodenitrificans TS-3 (ABN-TS) hydrolyzes the α-1,5-L-arabinofuranoside linkages of arabinan. In this study, the crystal structures of inactive ABN-TS mutants, D27A and D147N, were determined in complex with arabino-oligosaccharides. The crystal structures revealed that ABN-TS has at least six subsites in the deep V-shaped cleft formed across one face of the propeller structure. The structural features indicate that substrate recognition is profoundly influenced by the remote subsites as well as by the subsites surrounding the active center. The `open' structure of the substrate-binding cleft of the endo-acting ABN-TS is suitable for the random binding of several sugar units in polymeric substrates.

PubMed: 30511671
DOI: 10.1107/S2053230X18015947

実験手法

X-RAY DIFFRACTION (1.65 Å)