6A6X

The crystal structure of the Mtb MazE-MazF-mt9 complex

6A6X の概要

• エントリーDOI: 10.2210/pdb6a6x/pdb
• 分子名称: Probable endoribonuclease MazF7, Antitoxin MazE7, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: mycobacterium tuberculosis, toxin-antitoxin system, toxin
• 由来する生物種: Mycobacterium tuberculosis; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 47581.46

構造登録者

Xie, W.,Chen, R.,Tu, J. (登録日: 2018-06-29, 公開日: 2019-07-03, 最終更新日: 2023-11-22)

主引用文献

Chen, R.,Tu, J.,Tan, Y.,Cai, X.,Yang, C.,Deng, X.,Su, B.,Ma, S.,Liu, X.,Ma, P.,Du, C.,Xie, W.
Structural and Biochemical Characterization of the Cognate and Heterologous Interactions of the MazEF-mt9 TA System.
Acs Infect Dis., 5:1306-1316, 2019

PubMed Abstract: Toxin-antitoxin (TA) modules widely exist in bacteria, and their activities are associated with the persister phenotype of the pathogen (). causes tuberculosis, a contagious and severe airborne disease. There are 10 MazEF TA systems in that play important roles in stress adaptation. How the antitoxins antagonize toxins in or how the 10 TA systems crosstalk to each other are of interest, but the detailed molecular mechanisms are largely unclear. MazEF-mt9 is a unique member among the MazEF family due to its tRNase activity, which is usually carried out by the VapC toxins. Here, we present the cocrystal structure of the MazEF-mt9 complex at 2.7 Å. By characterizing the association mode between the TA pairs through various techniques, we found that MazF-mt9 bound not only its cognate antitoxin but also the noncognate antitoxin MazE-mt1, a phenomenon that could be also observed in vivo. Based on our structural and biochemical work, we propose that the cognate and heterologous interactions among different TA systems work together in vivo to relieve the toxicity of MazF-mt9 toward cells.

PubMed: 31267737
DOI: 10.1021/acsinfecdis.9b00001

実験手法

X-RAY DIFFRACTION (2.7 Å)