6A4W

AcrR from Mycobacterium tuberculosis

6A4W の概要

• エントリーDOI: 10.2210/pdb6a4w/pdb
• 分子名称: TetR family transcriptional regulator, SODIUM ION, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: transcription regulator, transcription
• 由来する生物種: Mycobacterium tuberculosis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 51549.31

構造登録者

Kang, S.M.,Kim, D.H. (登録日: 2018-06-21, 公開日: 2019-06-26, 最終更新日: 2023-11-22)

主引用文献

Kang, S.M.,Kim, D.H.,Jin, C.,Ahn, H.C.,Lee, B.J.
The crystal structure of AcrR from Mycobacterium tuberculosis reveals a one-component transcriptional regulation mechanism.
Febs Open Bio, 9:1713-1725, 2019

PubMed Abstract: Transcriptional regulator proteins are closely involved in essential survival strategies in bacteria. AcrR is a one-component allosteric repressor of the genes associated with lipid transport and antibiotic resistance. When fatty acid ligands bind to the C-terminal ligand-binding cavity of AcrR, a conformational change in the N-terminal operator-binding region of AcrR is triggered, which releases the repressed DNA and initiates transcription. This paper focuses on the structural transition mechanism of AcrR of Mycobacterium tuberculosis upon DNA and ligand binding. AcrR loses its structural integrity upon ligand-mediated structural alteration and bends toward the promoter DNA in a more compact form, initiating a rotational motion. Our functional characterization of AcrR and description of the ligand- and DNA-recognition mechanism may facilitate the discovery of new therapies for tuberculosis.

PubMed: 31369208
DOI: 10.1002/2211-5463.12710

実験手法

X-RAY DIFFRACTION (2.587 Å)