6A2I

Architectural roles of Cren7 in folding crenarchaeal chromatin filament

6A2I の概要

• エントリーDOI: 10.2210/pdb6a2i/pdb
• 関連するPDBエントリー: 6A2H
• 分子名称: Chromatin protein Cren7, DNA (5'-D(*CP*GP*TP*AP*GP*CP*TP*AP*AP*TP*TP*AP*GP*CP*TP*AP*CP*G)-3') (3 entities in total)
• 機能のキーワード: beta-sheet, dna binding protein-dna complex, crenarchaeal chromatin protein, dna binding protein/dna
• 由来する生物種: Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 24387.01

構造登録者

Zhang, Z.F.,Zhao, M.H.,Chen, Y.Y.,Wang, L.,Dong, Y.H.,Gong, Y.,Huang, L. (登録日: 2018-06-11, 公開日: 2019-01-16, 最終更新日: 2023-11-22)

主引用文献

Zhang, Z.,Zhao, M.,Chen, Y.,Wang, L.,Liu, Q.,Dong, Y.,Gong, Y.,Huang, L.
Architectural roles of Cren7 in folding crenarchaeal chromatin filament.
Mol. Microbiol., 111:556-569, 2019

PubMed Abstract: Archaea have evolved various strategies in chromosomal organization. While histone homologues exist in most archaeal phyla, Cren7 is a chromatin protein conserved in the Crenarchaeota. Here, we show that Cren7 preferentially binds DNA with AT-rich sequences over that with GC-rich sequences with a binding size of 6~7 bp. Structural studies of Cren7 in complex with either an 18-bp or a 20-bp double-stranded DNA fragment reveal that Cren7 binds to the minor groove of DNA as monomers in a head-to-tail manner. The neighboring Cren7 monomers are located on the opposite sides of the DNA duplex, with each introducing a single-step sharp kink by intercalation of the hydrophobic side chain of Leu28, bending the DNA into an S-shape conformation. A structural model for the chromatin fiber folded by Cren7 was established and verified by the analysis of cross-linked Cren7-DNA complexes by atomic force microscopy. Our results suggest that Cren7 differs significantly from Sul7, another chromatin protein conserved among Sulfolobus species, in both DNA binding and deformation. These data shed significant light on the strategy of chromosomal DNA organization in crenarchaea.

PubMed: 30499242
DOI: 10.1111/mmi.14173

実験手法

X-RAY DIFFRACTION (2.4 Å)