6A29

Crystal structure of PprA A139R mutant

Summary for 6A29

• Entry DOI: 10.2210/pdb6a29/pdb
• Descriptor: DNA repair protein PprA (2 entities in total)
• Functional Keywords: dna binding protein
• Biological source: Deinococcus radiodurans R1
• Total number of polymer chains: 8
• Total formula weight: 245313.82

Authors

Adachi, M.,Shibazaki, C.,Shimizu, R.,Arai, S.,Satoh, K.,Narumi, I.,Kuroki, R. (deposition date: 2018-06-09, release date: 2018-12-26, Last modification date: 2024-03-27)

Primary citation

Adachi, M.,Shimizu, R.,Shibazaki, C.,Satoh, K.,Fujiwara, S.,Arai, S.,Narumi, I.,Kuroki, R.
Extended structure of pleiotropic DNA repair-promoting protein PprA from Deinococcus radiodurans.
FASEB J., 33:3647-3658, 2019

PubMed Abstract: Pleiotropic protein promoting DNA repair A (PprA) is a key protein facilitating the extreme radiation resistance of Deinococcus radiodurans. PprA is a unique protein to the genus Deinococcus and exists as an oligomer ranging from a tetramer to an ∼100-mer depending on protein concentrations. Here, the X-ray crystal structure of PprA was determined to clarify how PprA confers radiation resistance. The tertiary structure of dimeric PprA was elucidated by using mutants obtained with random and site-directed mutagenesis methods (W183R and A139R); these mutants have disabled DNA binding and polymerization functions. Because the mutant A139R and W183R proteins have dimeric assemblies with 2 different interfaces (Interfaces 1 and 2), the linear and oligomerized PprA model was constructed as a left-handed face-to-face periodic screw structure. In addition, the linear structure in solution was confirmed by small-angle scattering experiments. The site-directed mutational analysis identified essential basic amino acids for DNA binding. These analytical data support the hypothesis that a complex assembly of PprA molecules, which are extended and have a screw structure, surrounds and stretches the DNA strand, acting as a novel guide to colocalize the DNA strands for efficient DNA repairs.-Adachi, M., Shimizu, R., Shibazaki, C., Satoh, K., Fujiwara, S., Arai, S., Narumi, I., Kuroki, R. Extended structure of pleiotropic DNA repair-promoting protein PprA from Deinococcus radiodurans.

PubMed: 30481062
DOI: 10.1096/fj.201801506R

Experimental method

X-RAY DIFFRACTION (2.399 Å)