6ZQV
Cryo-EM structure of mature Spondweni virus
Summary for 6ZQV
| Entry DOI | 10.2210/pdb6zqv/pdb |
| Related | 6ZQI 6ZQJ 6ZQU |
| EMDB information | 11364 11366 11370 11371 |
| Descriptor | Genome polyprotein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, 1,2-Distearoyl-sn-glycerophosphoethanolamine, ... (4 entities in total) |
| Functional Keywords | flavivirus, virus |
| Biological source | Spondweni virus More |
| Total number of polymer chains | 6 |
| Total formula weight | 193822.80 |
| Authors | Renner, M.,Dejnirattisai, W.,Carrique, L.,Serna Martin, I.,Karia, D.,Ilca, S.L.,Ho, S.F.,Kotecha, A.,Keown, J.R.,Mongkolsapaya, J.,Screaton, G.R.,Grimes, J.M. (deposition date: 2020-07-10, release date: 2021-01-20, Last modification date: 2024-11-20) |
| Primary citation | Renner, M.,Dejnirattisai, W.,Carrique, L.,Martin, I.S.,Karia, D.,Ilca, S.L.,Ho, S.F.,Kotecha, A.,Keown, J.R.,Mongkolsapaya, J.,Screaton, G.R.,Grimes, J.M. Flavivirus maturation leads to the formation of an occupied lipid pocket in the surface glycoproteins. Nat Commun, 12:1238-1238, 2021 Cited by PubMed Abstract: Flaviviruses such as Dengue (DENV) or Zika virus (ZIKV) assemble into an immature form within the endoplasmatic reticulum (ER), and are then processed by furin protease in the trans-Golgi. To better grasp maturation, we carry out cryo-EM reconstructions of immature Spondweni virus (SPOV), a human flavivirus of the same serogroup as ZIKV. By employing asymmetric localised reconstruction we push the resolution to 3.8 Å, enabling us to refine an atomic model which includes the crucial furin protease recognition site and a conserved Histidine pH-sensor. For direct comparison, we also solve structures of the mature forms of SPONV and DENV to 2.6 Å and 3.1 Å, respectively. We identify an ordered lipid that is present in only the mature forms of ZIKV, SPOV, and DENV and can bind as a consequence of rearranging amphipathic stem-helices of E during maturation. We propose a structural role for the pocket and suggest it stabilizes mature E. PubMed: 33623019DOI: 10.1038/s41467-021-21505-9 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.6 Å) |
Structure validation
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