6YI5

In-situ structure of the trimeric HEF from influenza C by flexible fitting into a cryo-ET map.

Summary for 6YI5

• Entry DOI: 10.2210/pdb6yi5/pdb
• EMDB information: 10810
• Descriptor: Hemagglutinin-esterase-fusion glycoprotein, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• Functional Keywords: receptor, esterase, fusion, influenza, viral protein
• Biological source: Influenza C virus (strain C/Johannesburg/1/1966); More
• Total number of polymer chains: 6
• Total formula weight: 206903.60

Authors

Halldorsson, S.,Rosenthal, P.B. (deposition date: 2020-03-31, release date: 2021-03-24, Last modification date: 2024-10-16)

Primary citation

Halldorsson, S.,Sader, K.,Turner, J.,Calder, L.J.,Rosenthal, P.B.
In situ structure and organization of the influenza C virus surface glycoprotein.
Nat Commun, 12:1694-1694, 2021

PubMed Abstract: The lipid-enveloped influenza C virus contains a single surface glycoprotein, the haemagglutinin-esterase-fusion (HEF) protein, that mediates receptor binding, receptor destruction, and membrane fusion at the low pH of the endosome. Here we apply electron cryotomography and subtomogram averaging to describe the structural basis for hexagonal lattice formation by HEF on the viral surface. The conformation of the glycoprotein in situ is distinct from the structure of the isolated trimeric ectodomain, showing that a splaying of the membrane distal domains is required to mediate contacts that form the lattice. The splaying of these domains is also coupled to changes in the structure of the stem region which is involved in membrane fusion, thereby linking HEF's membrane fusion conformation with its assembly on the virus surface. The glycoprotein lattice can form independent of other virion components but we show a major role for the matrix layer in particle formation.

PubMed: 33727554
DOI: 10.1038/s41467-021-21818-9

Experimental method

ELECTRON MICROSCOPY (9.1 Å)