6Y0O

isopenicillin N synthase in complex with ACV and Fe under anaerobic environment using FT-SSX methods

Summary for 6Y0O

• Entry DOI: 10.2210/pdb6y0o/pdb
• Related: 1BK0 1BLZ
• Descriptor: Anaerobic Fixed Target Structure of Isopenicillin N synthase in complex with Fe and ACV, SULFATE ION, L-D-(A-AMINOADIPOYL)-L-CYSTEINYL-D-VALINE, ... (5 entities in total)
• Functional Keywords: iron dependent oxygenase, penicillin biosynthesis, isopenicillin n, oxidoreductase
• Biological source: Aspergillus nidulans FGSC A4
• Total number of polymer chains: 1
• Total formula weight: 38175.24

Authors

Rabe, P.,Beale, J.H.,Lang, P.A.,Dirr, A.S.,Leissing, T.M.,Butryn, A.,Aller, P.,Kamps, J.J.A.G.,Axford, D.,McDonough, M.A.,Orville, A.M.,Owen, R.,Schofield, C.J. (deposition date: 2020-02-10, release date: 2020-09-09, Last modification date: 2024-01-24)

Primary citation

Rabe, P.,Beale, J.H.,Butryn, A.,Aller, P.,Dirr, A.,Lang, P.A.,Axford, D.N.,Carr, S.B.,Leissing, T.M.,McDonough, M.A.,Davy, B.,Ebrahim, A.,Orlans, J.,Storm, S.L.S.,Orville, A.M.,Schofield, C.J.,Owen, R.L.
Anaerobic fixed-target serial crystallography.
Iucrj, 7:901-912, 2020

PubMed Abstract: Cryogenic X-ray diffraction is a powerful tool for crystallographic studies on enzymes including oxygenases and oxidases. Amongst the benefits that cryo-conditions (usually employing a nitro-gen cryo-stream at 100 K) enable, is data collection of di-oxy-gen-sensitive samples. Although not strictly anaerobic, at low temperatures the vitreous ice conditions severely restrict O diffusion into and/or through the protein crystal. Cryo-conditions limit chemical reactivity, including reactions that require significant conformational changes. By contrast, data collection at room temperature imposes fewer restrictions on diffusion and reactivity; room-temperature serial methods are thus becoming common at synchrotrons and XFELs. However, maintaining an anaerobic environment for di-oxy-gen-dependent enzymes has not been explored for serial room-temperature data collection at synchrotron light sources. This work describes a methodology that employs an adaptation of the 'sheet-on-sheet' sample mount, which is suitable for the low-dose room-temperature data collection of anaerobic samples at synchrotron light sources. The method is characterized by easy sample preparation in an anaerobic glovebox, gentle handling of crystals, low sample consumption and preservation of a localized anaerobic environment over the timescale of the experiment (<5 min). The utility of the method is highlighted by studies with three X-ray-radiation-sensitive Fe(II)-containing model enzymes: the 2-oxoglutarate-dependent l-arginine hy-droxy-lase VioC and the DNA repair enzyme AlkB, as well as the oxidase isopenicillin N synthase (IPNS), which is involved in the biosynthesis of all penicillin and cephalosporin antibiotics.

PubMed: 32939282
DOI: 10.1107/S2052252520010374

Experimental method

X-RAY DIFFRACTION (2.2 Å)