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6WHV

GluN1b-GluN2B NMDA receptor in complex with SDZ 220-040 and L689,560, class 2

Summary for 6WHV
Entry DOI10.2210/pdb6whv/pdb
EMDB information21677
DescriptorGlutamate receptor ionotropic, NMDA 1, Glutamate receptor ionotropic, NMDA 2B, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
Functional Keywordsnmdars, ligand-gated ion channels, metal transport, ionotropic glutamate receptor, membrane protein, glun1 antagonist, glun2b antagonist
Biological sourceRattus norvegicus (Rat)
More
Total number of polymer chains4
Total formula weight416392.80
Authors
Chou, T.,Tajima, N.,Furukawa, H. (deposition date: 2020-04-08, release date: 2020-08-05)
Primary citationChou, T.H.,Tajima, N.,Romero-Hernandez, A.,Furukawa, H.
Structural Basis of Functional Transitions in Mammalian NMDA Receptors.
Cell, 182:357-371.e13, 2020
Cited by
PubMed Abstract: Excitatory neurotransmission meditated by glutamate receptors including N-methyl-D-aspartate receptors (NMDARs) is pivotal to brain development and function. NMDARs are heterotetramers composed of GluN1 and GluN2 subunits, which bind glycine and glutamate, respectively, to activate their ion channels. Despite importance in brain physiology, the precise mechanisms by which activation and inhibition occur via subunit-specific binding of agonists and antagonists remain largely unknown. Here, we show the detailed patterns of conformational changes and inter-subunit and -domain reorientation leading to agonist-gating and subunit-dependent competitive inhibition by providing multiple structures in distinct ligand states at 4 Å or better. The structures reveal that activation and competitive inhibition by both GluN1 and GluN2 antagonists occur by controlling the tension of the linker between the ligand-binding domain and the transmembrane ion channel of the GluN2 subunit. Our results provide detailed mechanistic insights into NMDAR pharmacology, activation, and inhibition, which are fundamental to the brain physiology.
PubMed: 32610085
DOI: 10.1016/j.cell.2020.05.052
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (4.05 Å)
Structure validation

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