6SMO

AntDE:AntF (apo): type II PKS acyl-carrier protein in complex with its ketosynthase bound to the hexaketide

Summary for 6SMO

• Entry DOI: 10.2210/pdb6smo/pdb
• Related: 1TQY 6SM4
• Descriptor: Acyl carrier protein, PKS_KS domain-containing protein, Ketoacyl_synth_N domain-containing protein, ... (7 entities in total)
• Functional Keywords: natural product biosynthesis, polyketides, minimal pks system, anthraquinone, chain elongation, catalysis, protein binding
• Biological source: Photorhabdus luminescens; More
• Total number of polymer chains: 7
• Total formula weight: 276168.93

Authors

Braeuer, A.,Zhou, Q.,Grammbitter, G.L.C.,Schmalhofer, M.,Ruehl, M.,Kaila, V.R.I.,Bode, H.,Groll, M. (deposition date: 2019-08-22, release date: 2020-05-27, Last modification date: 2024-01-24)

Primary citation

Brauer, A.,Zhou, Q.,Grammbitter, G.L.C.,Schmalhofer, M.,Ruhl, M.,Kaila, V.R.I.,Bode, H.B.,Groll, M.
Structural snapshots of the minimal PKS system responsible for octaketide biosynthesis.
Nat.Chem., 12:755-763, 2020

PubMed Abstract: Type II polyketide synthases (PKSs) are multi-enzyme complexes that produce secondary metabolites of medical relevance. Chemical backbones of such polyketides are produced by minimal PKS systems that consist of a malonyl transacylase, an acyl carrier protein and an α/β heterodimeric ketosynthase. Here, we present X-ray structures of all ternary complexes that constitute the minimal PKS system for anthraquinone biosynthesis in Photorhabdus luminescens. In addition, we characterize this invariable core using molecular simulations, mutagenesis experiments and functional assays. We show that malonylation of the acyl carrier protein is accompanied by major structural rearrangements in the transacylase. Principles of an ongoing chain elongation are derived from the ternary complex with a hexaketide covalently linking the heterodimeric ketosynthase with the acyl carrier protein. Our results for the minimal PKS system provide mechanistic understanding of PKSs and a fundamental basis for engineering PKS pathways for future applications.

PubMed: 32632186
DOI: 10.1038/s41557-020-0491-7

Experimental method

X-RAY DIFFRACTION (2.7 Å)