6RNF

Liquid Application Method for time-resolved Analyses (LAMA) by serial synchrotron crystallography, Xylose Isomerase 30 ms timepoint

Summary for 6RNF

Entry DOI	10.2210/pdb6rnf/pdb
Descriptor	Xylose isomerase, MAGNESIUM ION, alpha-D-glucopyranose, ... (4 entities in total)
Functional Keywords	isomerase, complex, diffusion, dynamics
Biological source	Streptomyces rubiginosus
Total number of polymer chains	1
Total formula weight	43512.06
Authors	Mehrabi, P.,Schulz, E.C.,Miller, R.J.D. (deposition date: 2019-05-08, release date: 2019-10-02, Last modification date: 2024-01-24)
Primary citation	Mehrabi, P.,Schulz, E.C.,Agthe, M.,Horrell, S.,Bourenkov, G.,von Stetten, D.,Leimkohl, J.P.,Schikora, H.,Schneider, T.R.,Pearson, A.R.,Tellkamp, F.,Miller, R.J.D. Liquid application method for time-resolved analyses by serial synchrotron crystallography. Nat.Methods, 16:979-982, 2019 Cited by PubMed Abstract: We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times. We demonstrate proof-of-principle binding of GlcNac to lysozyme, and resolve glucose binding and subsequent ring opening in a time-resolved study of xylose isomerase. PubMed: 31527838 DOI: 10.1038/s41592-019-0553-1 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.7 Å)

Structure validation