Summary for 6NY1
| Entry DOI | 10.2210/pdb6ny1/pdb |
| EMDB information | 8980 8994 8996 |
| Descriptor | CasX, DNA Non-target strand, gRNA, ... (4 entities in total) |
| Functional Keywords | casx, sgrna, target dna, crispr, rna binding protein-rna-dna complex, rna binding protein/rna/dna |
| Biological source | Deltaproteobacteria bacterium More |
| Total number of polymer chains | 4 |
| Total formula weight | 165666.00 |
| Authors | Liu, J.J.,Orlova, N.,Nogales, E.,Doudna, J.A. (deposition date: 2019-02-10, release date: 2019-02-27, Last modification date: 2024-03-13) |
| Primary citation | Liu, J.J.,Orlova, N.,Oakes, B.L.,Ma, E.,Spinner, H.B.,Baney, K.L.M.,Chuck, J.,Tan, D.,Knott, G.J.,Harrington, L.B.,Al-Shayeb, B.,Wagner, A.,Brotzmann, J.,Staahl, B.T.,Taylor, K.L.,Desmarais, J.,Nogales, E.,Doudna, J.A. CasX enzymes comprise a distinct family of RNA-guided genome editors. Nature, 566:218-223, 2019 Cited by PubMed Abstract: The RNA-guided CRISPR-associated (Cas) proteins Cas9 and Cas12a provide adaptive immunity against invading nucleic acids, and function as powerful tools for genome editing in a wide range of organisms. Here we reveal the underlying mechanisms of a third, fundamentally distinct RNA-guided genome-editing platform named CRISPR-CasX, which uses unique structures for programmable double-stranded DNA binding and cleavage. Biochemical and in vivo data demonstrate that CasX is active for Escherichia coli and human genome modification. Eight cryo-electron microscopy structures of CasX in different states of assembly with its guide RNA and double-stranded DNA substrates reveal an extensive RNA scaffold and a domain required for DNA unwinding. These data demonstrate how CasX activity arose through convergent evolution to establish an enzyme family that is functionally separate from both Cas9 and Cas12a. PubMed: 30718774DOI: 10.1038/s41586-019-0908-x PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (4.2 Å) |
Structure validation
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