6LYG
Cryo-EM structure of the calcium homeostasis modulator 1 channel
6LYG の概要
エントリーDOI | 10.2210/pdb6lyg/pdb |
EMDBエントリー | 30016 |
分子名称 | Calcium homeostasis modulator 1, 2-acetamido-2-deoxy-beta-D-glucopyranose (2 entities in total) |
機能のキーワード | octamer, membrane protein |
由来する生物種 | Danio rerio (Zebrafish) |
タンパク質・核酸の鎖数 | 8 |
化学式量合計 | 331478.82 |
構造登録者 | |
主引用文献 | Ren, Y.,Wen, T.,Xi, Z.,Li, S.,Lu, J.,Zhang, X.,Yang, X.,Shen, Y. Cryo-EM structure of the calcium homeostasis modulator 1 channel. Sci Adv, 6:eaba8161-eaba8161, 2020 Cited by PubMed Abstract: Calcium homeostasis modulator 1 (CALHM1) is a voltage-gated ATP release channel that plays an important role in neural gustatory signaling and the pathogenesis of Alzheimer's disease. Here, we present a cryo-electron microscopy structure of full-length Ca-free CALHM1 from Danio rerio at an overall resolution of 3.1 Å. Our structure reveals an octameric architecture with a wide pore diameter of ~20 Å, presumably representing the active conformation. The overall structure is substantially different from that of the isoform CALHM2, which forms both undecameric hemichannels and gap junctions. The N-terminal small helix folds back to the pore and forms an antiparallel interaction with transmembrane helix 1. Structural analysis revealed that the extracellular loop 1 region within the dimer interface may contribute to oligomeric assembly. A positive potential belt inside the pore was identified that may modulate ion permeation. Our structure offers insights into the assembly and gating mechanism of the CALHM1 channel. PubMed: 32832630DOI: 10.1126/sciadv.aba8161 主引用文献が同じPDBエントリー |
実験手法 | ELECTRON MICROSCOPY (3.1 Å) |
構造検証レポート
検証レポート(詳細版)
をダウンロード
