6HOS

Structure of the KpFlo2 adhesin domain in complex with glycerol

Summary for 6HOS

• Entry DOI: 10.2210/pdb6hos/pdb
• Descriptor: BA75_04148T0, Expression tag from chain B, or symmetry related chain, CALCIUM ION, ... (8 entities in total)
• Functional Keywords: c-type lectin, komagataella pastoris, cell surface, cell adhesion
• Biological source: Komagataella pastoris (Yeast); More
• Total number of polymer chains: 3
• Total formula weight: 58013.45

Authors

Essen, L.-O.,Kock, M.,Veelders, M. (deposition date: 2018-09-18, release date: 2018-10-24, Last modification date: 2024-11-13)

Primary citation

Kock, M.,Bruckner, S.,Wozniak, N.,Maestre-Reyna, M.,Veelders, M.,Schlereth, J.,Mosch, H.U.,Essen, L.O.
Structural and Functional Characterization of PA14/Flo5-Like Adhesins FromKomagataella pastoris.
Front Microbiol, 9:2581-2581, 2018

PubMed Abstract: Cell-cell and cell-substrate based adhesion of yeasts are major determinants of their adoption of different life styles. Genome-mining of ascomycetous GPI-anchored cell wall proteins with lectin-like PA14 domains identified a unique class of putative adhesins in the clade of methylotrophic yeasts, many of which are known to colonize plants and insects involving yet unknown adhesion mechanisms. Here, we report the functional and structural analysis of two of its members: Flo1 (=Cea1), that is highly specific for terminal -acetylglucosamine moieties, and Flo2, which represents an orphan lectin with intact binding site but unknown specificity. Crystal structures of the Cea1 adhesion domain complexed to -acetylglucosamine and ,'-diacetylchitobiose reveal a Ca-dependent binding mode that differs from other members of the PA14/Flo5 adhesin family. Heterologous expression of Cea1A in promotes cellular adhesion to non-reducing ends of non-crystalline chitin. Overall, our data suggest that high-affinity recognition of β-GlcNAc-capped glycans by Cea1 enable species to interact with surface cues present in fungi and insects.

PubMed: 30425696
DOI: 10.3389/fmicb.2018.02581

Experimental method

X-RAY DIFFRACTION (2.15 Å)