6EV2

Crystal structure of antibody against schizophyllan in complex with laminarihexaose

Summary for 6EV2

• Entry DOI: 10.2210/pdb6ev2/pdb
• Related PRD ID: PRD_900024
• Descriptor: Heavy chain, Light chain, beta-D-glucopyranose-(1-3)-beta-D-glucopyranose, ... (5 entities in total)
• Functional Keywords: antibody, fab, schizophyllan, laminarihexaose, immune system
• Biological source: Mus musculus; More
• Total number of polymer chains: 8
• Total formula weight: 189344.81

Authors

Sung, K.H.,Josewski, J.,Duebel, S.,Blankenfeldt, W.,Rau, U. (deposition date: 2017-11-01, release date: 2018-09-26, Last modification date: 2024-10-23)

Primary citation

Sung, K.H.,Josewski, J.,Dubel, S.,Blankenfeldt, W.,Rau, U.
Structural insights into antigen recognition of an anti-beta-(1,6)-beta-(1,3)-D-glucan antibody.
Sci Rep, 8:13652-13652, 2018

PubMed Abstract: Schizophyllan (SCH) is a high molecular weight homopolysaccharide composed of a β-(1,3)-D-glucan main chain with branching β-(1,6)-bound D-glucose residues. It forms triple helices that are highly stable towards heat and extreme pH, which provides SCH with interesting properties for industrial and medical applications. The recombinant anti-SCH antibody JoJ48C11 recognizes SCH and related β-(1,6)-branched β-(1,3)-D-glucans, but details governing its specificity are not known. Here, we fill this gap by determining crystal structures of the antigen binding fragment (Fab) of JoJ48C11 in the apo form and in complex with the unbranched β-(1,3)-D-glucose hexamer laminarihexaose 3.0 and 2.4 Å resolution, respectively. Together with docking studies, this allowed construction of a JoJ48C11/triple-helical SCH complex, leading to the identification of eight amino acid residues of JoJ48C11 (Tyr27, His35, Trp47, Trp100, Asp105; Asp49, Lys52, Trp90) that contribute to the recognition of glucose units from all three chains of the SCH triple helix. The importance of these amino acids was confirmed by mutagenesis and ELISA-based analysis. Our work provides an explanation for the specific recognition of triple-helical β-(1,6)-branched β-(1,3)-D-glucans by JoJ48C11 and provides another structure example for anti-carbohydrate antibodies.

PubMed: 30209318
DOI: 10.1038/s41598-018-31961-x

Experimental method

X-RAY DIFFRACTION (2.403 Å)