6D3B

INFLUENZA VIRUS NEURAMINIDASE SUBTYPE N9 (TERN) APO FORM

Summary for 6D3B

• Entry DOI: 10.2210/pdb6d3b/pdb
• Related: 6CRD 7NN9
• Descriptor: Neuraminidase, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• Functional Keywords: neuraminidase, sialidase, hydrolase(o-glucosyl), hydrolase, viral protein
• Biological source: Influenza A virus (strain A/Tern/Australia/G70C/1975 H11N9)
• Total number of polymer chains: 1
• Total formula weight: 46490.45

Authors

Streltsov, V.A.,Schmidt, P.,McKimm-Breschkin, J. (deposition date: 2018-04-15, release date: 2019-01-23, Last modification date: 2023-10-04)

Primary citation

Streltsov, V.A.,Schmidt, P.M.,McKimm-Breschkin, J.L.
Structure of an Influenza A virus N9 neuraminidase with a tetrabrachion-domain stalk.
Acta Crystallogr.,Sect.F, 75:89-97, 2019

PubMed Abstract: The influenza neuraminidase (NA) is a homotetramer with head, stalk, transmembrane and cytoplasmic regions. The structure of the NA head with a stalk has never been determined. The NA head from an N9 subtype influenza A virus, A/tern/Australia/G70C/1975 (H1N9), was expressed with an artificial stalk derived from the tetrabrachion (TB) tetramerization domain from Staphylothermus marinus. The NA was successfully crystallized both with and without the TB stalk, and the structures were determined to 2.6 and 2.3 Å resolution, respectively. Comparisons of the two NAs with the native N9 NA structure from egg-grown virus showed that the artificial TB stalk maintained the native NA head structure, supporting previous biological observations.

PubMed: 30713159
DOI: 10.1107/S2053230X18017892

Experimental method

X-RAY DIFFRACTION (1.4 Å)