6A56

AJLec from the Sea Anemone Anthopleura japonica

Summary for 6A56

• Entry DOI: 10.2210/pdb6a56/pdb
• Related PRD ID: PRD_900004
• Descriptor: AJLec, beta-D-galactopyranose-(1-4)-beta-D-glucopyranose, CALCIUM ION, ... (5 entities in total)
• Functional Keywords: ajlec, lectin, sea anemone, gyractis japonica, sugar binding protein
• Biological source: Anthopleura japonica
• Total number of polymer chains: 2
• Total formula weight: 38040.77

Authors

Unno, H.,Hatakeyama, T. (deposition date: 2018-06-22, release date: 2018-07-25, Last modification date: 2024-10-23)

Primary citation

Unno, H.,Nakamura, A.,Mori, S.,Goda, S.,Yamaguchi, K.,Hiemori, K.,Tateno, H.,Hatakeyama, T.
Identification, Characterization, and X-ray Crystallographic Analysis of a Novel Type of Lectin AJLec from the Sea Anemone Anthopleura japonica.
Sci Rep, 8:11516-11516, 2018

PubMed Abstract: A novel galactose-specific lectin, AJLec (18.5 kDa), was isolated from the sea anemone, Anthopleura japonica. AJLec was characterized using the hemagglutination assay, isothermal titration calorimetry (ITC), and glycoconjugate microarray analysis and we found that AJLec has a specificity for galactose monomers and β-linked terminal galactose residues in complex carbohydrates, but not for N-acetylgalactosamine (GalNAc), which is commonly recognized by galactose-binding lectins. The primary structure of AJLec did not show homology with known lectins, and a crystal structural analysis also revealed a unique homodimeric structure. The crystal structure of AJLec complexed with lactose was solved by measuring the sulfur single-wavelength anomalous diffraction (S-SAD) phasing with an in-house Cu Kα source method. This analysis revealed that the galactose residue in lactose was recognized via its O2, O3, and O4 hydroxyl groups and ring oxygen by calcium coordination and two hydrogen bonds with residues in the carbohydrate-binding site, which demonstrated strict specificity for the β-linked terminal galactose in this lectin.

PubMed: 30068923
DOI: 10.1038/s41598-018-29498-0

Experimental method

X-RAY DIFFRACTION (1.2 Å)