5ZZ8

Structure of the Herpes simplex virus type 2 C-capsid with capsid-vertex-specific component

This is a non-PDB format compatible entry.

Summary for 5ZZ8

• Entry DOI: 10.2210/pdb5zz8/pdb
• EMDB information: 6976
• Descriptor: VP19C, VP23, Major capsid protein, ... (7 entities in total)
• Functional Keywords: herpes simplex virus 2, capsid, capsid-vertex-specific component, structural protein
• Biological source: Human herpesvirus 2 (HHV-2); More
• Total number of polymer chains: 51
• Total formula weight: 4032164.48

Authors

Wang, J.L.,Yuan, S.,Zhu, D.J.,Tang, H.,Wang, N.,Chen, W.Y.,Gao, Q.,Li, Y.H.,Wang, J.Z.,Liu, H.R.,Zhang, X.Z.,Rao, Z.H.,Wang, X.X. (deposition date: 2018-05-31, release date: 2018-10-10, Last modification date: 2019-11-06)

Primary citation

Wang, J.,Yuan, S.,Zhu, D.,Tang, H.,Wang, N.,Chen, W.,Gao, Q.,Li, Y.,Wang, J.,Liu, H.,Zhang, X.,Rao, Z.,Wang, X.
Structure of the herpes simplex virus type 2 C-capsid with capsid-vertex-specific component.
Nat Commun, 9:3668-3668, 2018

PubMed Abstract: Herpes simplex viruses (HSVs) cause human oral and genital ulcer diseases. Patients with HSV-2 have a higher risk of acquiring a human immunodeficiency virus infection. HSV-2 is a member of the α-herpesvirinae subfamily that together with the β- and γ-herpesvirinae subfamilies forms the Herpesviridae family. Here, we report the cryo-electron microscopy structure of the HSV-2 C-capsid with capsid-vertex-specific component (CVSC) that was determined at 3.75 Å using a block-based reconstruction strategy. We present atomic models of multiple conformers for the capsid proteins (VP5, VP23, VP19C, and VP26) and CVSC. Comparison of the HSV-2 homologs yields information about structural similarities and differences between the three herpesviruses sub-families and we identify α-herpesvirus-specific structural features. The hetero-pentameric CVSC, consisting of a UL17 monomer, a UL25 dimer and a UL36 dimer, is bound tightly by a five-helix bundle that forms extensive networks of subunit contacts with surrounding capsid proteins, which reinforce capsid stability.

PubMed: 30201968
DOI: 10.1038/s41467-018-06078-4

Experimental method

ELECTRON MICROSCOPY (3.75 Å)