5ZZ7

Redox-sensing transcriptional repressor Rex

5ZZ7 の概要

• エントリーDOI: 10.2210/pdb5zz7/pdb
• 分子名称: Redox-sensing transcriptional repressor Rex 1, 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: redox sensing, transcription factor, gene regulation
• 由来する生物種: Thermotoga maritima MSB8
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47488.36

構造登録者

Park, Y.W.,Jang, Y.Y.,Joo, H.K.,Lee, J.Y. (登録日: 2018-05-30, 公開日: 2018-11-07, 最終更新日: 2024-03-27)

主引用文献

Park, Y.W.,Jang, Y.Y.,Joo, H.K.,Lee, J.Y.
Structural Analysis of Redox-sensing Transcriptional Repressor Rex from Thermotoga maritima
Sci Rep, 8:13244-13244, 2018

PubMed Abstract: The cellular redox state of organisms continues to fluctuate during the metabolism. All organisms have various sensors that help detect and adapt to changes in the redox state. Nicotinamide adenine dinucleotides (NAD/NADH), which are involved in various cellular metabolic activities as cofactors, have been revealed as the key molecules sensing the intra-cellular redox state. The Rex family members are well conserved transcriptional repressors that regulate the expression of respiratory genes by sensing the redox state according to the intra-cellular NAD/NADH balance. Herein, we reported crystal structures of apo and NAD- and NADH-bound forms of Rex from Thermotoga maritima to analyse the structural basis of transcriptional regulation depending on either NAD or NADH binding. The different orientation of the reduced nicotinamide group to helix α9 caused the rearrangement of N-terminal DNA binding domain, thus resulting in closed form of Rex to dissociate from cognate DNA. The structural data of Rex from T. maritima also support the previous redox-sensing mechanism models of Rex homologues.

PubMed: 30185822
DOI: 10.1038/s41598-018-31676-z

実験手法

X-RAY DIFFRACTION (2.45 Å)