5ZWN
Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.3 angstrom (Part II: U1 snRNP region)
Summary for 5ZWN
| Entry DOI | 10.2210/pdb5zwn/pdb |
| Related | 5ZWM |
| EMDB information | 6972 6973 |
| Descriptor | pre-mRNA, Small nuclear ribonucleoprotein-associated protein B, Small nuclear ribonucleoprotein Sm D1, ... (21 entities in total) |
| Functional Keywords | spliceosme, assemply, pre-b complex, u1 snrnp, splicing |
| Biological source | Saccharomyces cerevisiae S288c More |
| Total number of polymer chains | 20 |
| Total formula weight | 802383.46 |
| Authors | |
| Primary citation | Bai, R.,Wan, R.,Yan, C.,Lei, J.,Shi, Y. Structures of the fully assembledSaccharomyces cerevisiaespliceosome before activation Science, 360:1423-1429, 2018 Cited by PubMed Abstract: The precatalytic spliceosome (B complex) is preceded by the pre-B complex. Here we report the cryo-electron microscopy structures of the pre-B and B complexes at average resolutions of 3.3 to 4.6 and 3.9 angstroms, respectively. In the pre-B complex, the duplex between the 5' splice site (5'SS) and U1 small nuclear RNA (snRNA) is recognized by Yhc1, Luc7, and the Sm ring. In the B complex, U1 small nuclear ribonucleoprotein is dissociated, the 5'-exon-5'SS sequences are translocated near U6 snRNA, and three B-specific proteins may orient the precursor messenger RNA. In both complexes, U6 snRNA is anchored to loop I of U5 snRNA, and the duplex between the branch point sequence and U2 snRNA is recognized by the SF3b complex. Structural analysis reveals the mechanism of assembly and activation for the yeast spliceosome. PubMed: 29794219DOI: 10.1126/science.aau0325 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.4 Å) |
Structure validation
Download full validation report
