5ZU3

Effect of mutation (R554K) on FAD modification in Aspergillus oryzae RIB40formate oxidase

Summary for 5ZU3

• Entry DOI: 10.2210/pdb5zu3/pdb
• Descriptor: Formate oxidase, [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-(8-formyl-7-methyl-2,4-dioxo-3,4-dihydrobenzo[g]pteridin-10(2H)-yl)-2,3,4-trihydroxypentyl dihydrogen diphosphate, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (5 entities in total)
• Functional Keywords: formate oxidase, 8-formyl-fad, effect of mutation, oxidoreductase
• Biological source: Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
• Total number of polymer chains: 3
• Total formula weight: 195914.73

Authors

Mikami, B.,Uchida, H.,Doubayashi, D. (deposition date: 2018-05-06, release date: 2019-05-22, Last modification date: 2024-03-27)

• Primary citation: Doubayashi, D.,Oki, M.,Mikami, B.,Uchida, H.; The microenvironment surrounding FAD mediates its conversion to 8-formyl-FAD in Aspergillus oryzae RIB40 formate oxidase.; J.Biochem., 166:67-75, 2019; PubMed: 30715389; DOI: 10.1093/jb/mvz009

Experimental method

X-RAY DIFFRACTION (2.4 Å)