5ZTC

Apo structure of TetR family transcription regulator Lmo2088 of Listeria monocytogenes EGDe

5ZTC の概要

• エントリーDOI: 10.2210/pdb5ztc/pdb
• 分子名称: Lmo2088 protein, PENTAETHYLENE GLYCOL (3 entities in total)
• 機能のキーワード: tetr family, transcription
• 由来する生物種: Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 48353.16

構造登録者

Samad, A.,Jin, T. (登録日: 2018-05-02, 公開日: 2019-06-12, 最終更新日: 2024-03-27)

主引用文献

Samad, A.,Li, Y.,Zhang, C.,Chen, F.,Zeng, W.,Fan, X.,Jin, T.
X-ray crystal structure of putative transcription regulator lmo2088 from Listeria monocytogenes.
Biochem.Biophys.Res.Commun., 520:434-440, 2019

PubMed Abstract: Listeria monocytogenes is a gram-positive food borne pathogen. The lmo2088 belongs to the TetR family of transcriptional regulators from L. monocytogenes. These transcriptional factors regulate multidrug resistance transporters in L. monocytogenes. Here, we report native protein crystal structure of lmo2088 at a resolution of 1.7 Å. Lmo2088 comprises of an N-terminal DNA binding domain and a variable C-terminal effector binding domain. Furthermore, we identified specific consensus sequences selected by systematic evolution of ligands by exponential enrichment in vitro. The specific binding of lmo2088 with DNA consensus sequence was validated by electrophoretic mobility shift assay, fluorescence polarization and isothermal titration calorimetry. We speculate that the structure of lmo2088 might provide an insight into the regulatory function of lmo2088 of L. monocytogenes.

PubMed: 31607473
DOI: 10.1016/j.bbrc.2019.10.033

実験手法

X-RAY DIFFRACTION (1.7 Å)