5ZRQ

Crystal structure of PET-degrading cutinase Cut190 S176A/S226P/R228S mutant in Zn(2+)-bound state

5ZRQ の概要

• エントリーDOI: 10.2210/pdb5zrq/pdb
• 関連するPDBエントリー: 5ZNO 5ZRR 5ZRS
• 分子名称: Alpha/beta hydrolase family protein, ZINC ION, CALCIUM ION, ... (6 entities in total)
• 機能のキーワード: polyesterase, alpha/beta-hydrolase fold, protein engineering, thermostability, hydrolase
• 由来する生物種: Saccharomonospora viridis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29949.13

構造登録者

Numoto, N.,Kamiya, N.,Bekker, G.J.,Yamagami, Y.,Inaba, S.,Ishii, K.,Uchiyama, S.,Kawai, F.,Ito, N.,Oda, M. (登録日: 2018-04-25, 公開日: 2018-09-12, 最終更新日: 2023-11-22)

• 主引用文献: Numoto, N.,Kamiya, N.,Bekker, G.J.,Yamagami, Y.,Inaba, S.,Ishii, K.,Uchiyama, S.,Kawai, F.,Ito, N.,Oda, M.; Structural Dynamics of the PET-Degrading Cutinase-like Enzyme from Saccharomonospora viridis AHK190 in Substrate-Bound States Elucidates the Ca2+-Driven Catalytic Cycle.; Biochemistry, 57:5289-5300, 2018; PubMed: 30110540; DOI: 10.1021/acs.biochem.8b00624

実験手法

X-RAY DIFFRACTION (1.12 Å)