5ZNO
Crystal structure of PET-degrading cutinase Cut190 S176A/S226P/R228S/ mutant in Ca(2+)-bound state
Summary for 5ZNO
Entry DOI | 10.2210/pdb5zno/pdb |
Descriptor | Alpha/beta hydrolase family protein, CALCIUM ION, GLYCEROL, ... (4 entities in total) |
Functional Keywords | polyesterase, alpha/beta-hydrolase fold, protein engineering, thermostability, hydrolase |
Biological source | Saccharomonospora viridis |
Total number of polymer chains | 2 |
Total formula weight | 59128.33 |
Authors | Numoto, N.,Inaba, S.,Yamagami, Y.,Kamiya, N.,Bekker, G.J.,Ishii, K.,Uchiyama, S.,Kawai, F.,Ito, N.,Oda, M. (deposition date: 2018-04-10, release date: 2018-09-12, Last modification date: 2023-11-22) |
Primary citation | Numoto, N.,Kamiya, N.,Bekker, G.J.,Yamagami, Y.,Inaba, S.,Ishii, K.,Uchiyama, S.,Kawai, F.,Ito, N.,Oda, M. Structural Dynamics of the PET-Degrading Cutinase-like Enzyme from Saccharomonospora viridis AHK190 in Substrate-Bound States Elucidates the Ca2+-Driven Catalytic Cycle. Biochemistry, 57:5289-5300, 2018 Cited by PubMed: 30110540DOI: 10.1021/acs.biochem.8b00624 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.60264346898 Å) |
Structure validation
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