5ZIM

Crystal structure of bacteriorhodopsin at 1.25 A resolution

5ZIM の概要

• エントリーDOI: 10.2210/pdb5zim/pdb
• 分子名称: Bacteriorhodopsin, RETINAL, 2,3-DI-PHYTANYL-GLYCEROL, ... (4 entities in total)
• 機能のキーワード: proton pump, membrane protein, proton transport
• 由来する生物種: Halobacterium salinarum (Halobacterium halobium)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27887.60

構造登録者

Hasegawa, N.,Jonotsuka, H.,Miki, K.,Takeda, K. (登録日: 2018-03-16, 公開日: 2018-10-10, 最終更新日: 2023-11-22)

主引用文献

Hasegawa, N.,Jonotsuka, H.,Miki, K.,Takeda, K.
X-ray structure analysis of bacteriorhodopsin at 1.3 angstrom resolution.
Sci Rep, 8:13123-13123, 2018

PubMed Abstract: Bacteriorhodopsin (bR) of Halobacterium salinarum is a membrane protein that acts as a light-driven proton pump. bR and its homologues have recently been utilized in optogenetics and other applications. Although the structures of those have been reported so far, the resolutions are not sufficient for elucidation of the intrinsic structural features critical to the color tuning and ion pumping properties. Here we report the accurate crystallographic analysis of bR in the ground state. The influence of X-rays was suppressed by collecting the data under a low irradiation dose at 15 K. Consequently, individual atoms could be separately observed in the electron density map at better than 1.3 Å resolution. Residues from Thr5 to Ala233 were continuously constructed in the model. The twist of the retinal polyene was determined to be different from those in the previous models. Two conformations were observed for the proton release region. We discuss the meaning of these fine structural features.

PubMed: 30177765
DOI: 10.1038/s41598-018-31370-0

実験手法

X-RAY DIFFRACTION (1.25 Å)