5ZHW

VanYB in complex with D-Alanine-D-Alanine

5ZHW の概要

• エントリーDOI: 10.2210/pdb5zhw/pdb
• 関連するPDBエントリー: 5ZHF
• 分子名称: D-alanyl-D-alanine carboxypeptidase, COPPER (II) ION, D-ALANINE, ... (6 entities in total)
• 機能のキーワード: vancomycin resistance, peptidase, metallopeptidase family m15, hydrolase
• 由来する生物種: Enterococcus faecalis V583
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 52714.18

構造登録者

Kim, H.S.,Hahn, H. (登録日: 2018-03-13, 公開日: 2018-09-05, 最終更新日: 2024-10-23)

主引用文献

Kim, H.S.,Hahn, H.,Kim, J.,Jang, D.M.,Lee, J.Y.,Back, J.M.,Im, H.N.,Kim, H.,Han, B.W.,Suh, S.W.
Structural basis for the substrate recognition of peptidoglycan pentapeptides by Enterococcus faecalis VanYB.
Int. J. Biol. Macromol., 119:335-344, 2018

PubMed Abstract: Vancomycin resistance in Enterococci and its transfer to methicillin-resistant Staphylococcus aureus are challenging problems in health care institutions worldwide. High-level vancomycin resistance is conferred by acquiring either transposable elements of the VanA or VanB type. Enterococcus faecalis VanY in the VanB-type operon is a d,d-carboxypeptidase that recognizes the peptidyl-d-Ala-d-Ala extremity of peptidoglycan and hydrolyses the terminal d-Ala on the extracellular side of the cell wall, thereby increasing the level of glycopeptide antibiotics resistance. However, at the molecular level, it remains unclear how VanY manipulates peptidoglycan peptides for vancomycin resistance. In this study, we have determined the crystal structures of E. faecalis VanY in the d-Ala-d-Ala-bound, d-Ala-bound, and -unbound states. The interactions between VanY and d-Ala-d-Ala observed in the crystal provide the molecular basis for the recognition of peptidoglycan substrates by VanY. Moreover, comparisons with the related VanX and VanXY enzymes reveal distinct structural features of E. faecalis VanY around the active-site cleft, thus shedding light on its unique substrate specificity. Our results could serve as the foundation for unravelling the molecular mechanism of vancomycin resistance and for developing novel antibiotics against the vancomycin-resistant Enterococcus species.

PubMed: 30016658
DOI: 10.1016/j.ijbiomac.2018.07.081

実験手法

X-RAY DIFFRACTION (2.18 Å)