5ZDP

Crystal structure of cyanide-insensitive alternative oxidase from Trypanosoma brucei with ferulenol

5ZDP の概要

• エントリーDOI: 10.2210/pdb5zdp/pdb
• 分子名称: Alternative oxidase, mitochondrial, FE (III) ION, HYDROXIDE ION, ... (6 entities in total)
• 機能のキーワード: membrane bound diiron protein, membrane protein
• 由来する生物種: Trypanosoma brucei brucei
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 152681.91

構造登録者

Shiba, T.,Inaoka, D.K.,Takahashi, G.,Tsuge, C.,Kido, Y.,Young, L.,Ueda, S.,Balogun, E.O.,Nara, T.,Honma, T.,Tanaka, A.,Inoue, M.,Saimoto, H.,Harada, S.,Moore, A.L.,Kita, K. (登録日: 2018-02-23, 公開日: 2019-02-27, 最終更新日: 2023-11-22)

主引用文献

Shiba, T.,Inaoka, D.K.,Takahashi, G.,Tsuge, C.,Kido, Y.,Young, L.,Ueda, S.,Balogun, E.O.,Nara, T.,Honma, T.,Tanaka, A.,Inoue, M.,Saimoto, H.,Harada, S.,Moore, A.L.,Kita, K.
Insights into the ubiquinol/dioxygen binding and proton relay pathways of the alternative oxidase.
Biochim Biophys Acta Bioenerg, 1860:375-382, 2019

PubMed Abstract: The alternative oxidase (AOX) is a monotopic diiron carboxylate protein which catalyzes the four-electron reduction of dioxygen to water by ubiquinol. Although we have recently determined the crystal structure of Trypanosoma brucei AOX (TAO) in the presence and absence of ascofuranone (AF) derivatives (which are potent mixed type inhibitors) the mechanism by which ubiquinol and dioxygen binds to TAO remain inconclusive. In this article, ferulenol was identified as the first competitive inhibitor of AOX which has been used to probe the binding of ubiquinol. Surface plasmon resonance reveals that AF is a quasi-irreversible inhibitor of TAO whilst ferulenol binding is completely reversible. The structure of the TAO-ferulenol complex, determined at 2.7 Å, provided insights into ubiquinol binding and has also identified a potential dioxygen molecule bound in a side-on conformation to the diiron center for the first time.

PubMed: 30910528
DOI: 10.1016/j.bbabio.2019.03.008

実験手法

X-RAY DIFFRACTION (2.71 Å)