5ZCT

The crystal structure of the poly-alpha-L-glutamate peptides synthetase RimK at 2.05 angstrom resolution.

5ZCT の概要

• エントリーDOI: 10.2210/pdb5zct/pdb
• 分子名称: Ribosomal protein S6--L-glutamate ligase, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: l-amino acid ligase, ligase
• 由来する生物種: Escherichia coli (strain K12)
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 270422.92

構造登録者

Arimura, Y.,Kono, T.,Kino, K.,Kurumizaka, H. (登録日: 2018-02-20, 公開日: 2018-07-25, 最終更新日: 2025-09-17)

主引用文献

Arimura, Y.,Kono, T.,Kino, K.,Kurumizaka, H.
Structural polymorphism of the Escherichia coli poly-alpha-L-glutamate synthetase RimK
Acta Crystallogr F Struct Biol Commun, 74:385-390, 2018

PubMed Abstract: Bacterial RimK is an enzyme that catalyzes the polyglutamylation of the C-terminus of ribosomal protein S6 and the synthesis of poly-α-L-glutamate peptides using L-glutamic acid. In the present study, the crystal structure of the Escherichia coli RimK protein complexed with the ATP analogue AMP-PNP was determined at 2.05 Å resolution. Two different conformations of RimK, closed and open forms, were observed in the crystals. The structural polymorphism revealed in this study provided important information to understand the mechanism by which RimK catalyzes the synthesis of poly-α-L-glutamate peptides and the polyglutamylation of ribosomal protein S6.

PubMed: 29969101
DOI: 10.1107/S2053230X18007689

実験手法

X-RAY DIFFRACTION (2.05 Å)