5ZCN

Solution NMR structure of a new lasso peptide brevunsin

5ZCN の概要

• エントリーDOI: 10.2210/pdb5zcn/pdb
• NMR情報: BMRB: 36167
• 分子名称: brevunsin (1 entity in total)
• 機能のキーワード: lasso peptide, sphingomonas subterranea, brevunsin, unknown function
• 由来する生物種: Brevundimonas diminuta
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2253.47

構造登録者

Hemmi, H.,Kodani, S.,Miyake, Y.,Kaweewan, I.,Nakagawa, H. (登録日: 2018-02-19, 公開日: 2018-10-17, 最終更新日: 2024-10-09)

主引用文献

Kodani, S.,Hemmi, H.,Miyake, Y.,Kaweewan, I.,Nakagawa, H.
Heterologous production of a new lasso peptide brevunsin in Sphingomonas subterranea
J. Ind. Microbiol. Biotechnol., 45:983-992, 2018

PubMed Abstract: A shuttle vector pHSG396Sp was constructed to perform gene expression using Sphingomonas subterranea as a host. A new lasso peptide biosynthetic gene cluster, derived from Brevundimonas diminuta, was amplified by PCR and integrated to afford a expression vector pHSG396Sp-12697L. The new lasso peptide brevunsin was successfully produced by S. subterranea, harboring the expression vector, with a high production yield (10.2 mg from 1 L culture). The chemical structure of brevunsin was established by NMR and MS/MS experiments. Based on the information obtained from the NOE experiment, the three-dimensional structure of brevunsin was determined, which indicated that brevunsin possessed a typical lasso structure. This expression vector system provides a new heterologous production method for unexplored lasso peptides that are encoded by bacterial genomes.

PubMed: 30191430
DOI: 10.1007/s10295-018-2077-6

実験手法

SOLUTION NMR